Human OH Vitamin D ELISA KIT

$566.00

  • Model: EF007396
  • 100 Units in Stock
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OH Vitamin D ELISA KIT|Human

Catalog  EF007396

Packing 48T/96T

Reactivity Human

Detect Range 1.563-100ng/ml

OH Vitamin D ELISA KIT Sensitivity< 0.938ng/ml

Application OH Vitamin D ELISA KIT|Human  is for detecting the concentration of Human OH Vitamin D in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

 

Principle of OH Vitamin D ELISA KIT|Human

The microtiter plate provided in OH Vitamin D ELISA KIT|Human has been pre-coated with an Human OH Vitamin D antibody specific to Human OH Vitamin D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human OH Vitamin Dantibody preparation specific for Human OH Vitamin D then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human OH Vitamin D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human OH Vitamin Din the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

*Please noted that OH Vitamin D ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!

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