Human Oviduct- specific glycoprotein, OVGP1 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

OVGP,OVGP1,CHIT5; EGP; MUC9; OGP; estrogen-dependent oviduct protein; mucin 9; mucin-9; oviduct glycoprotein; oviduct-specific glycoprotein; oviductin; oviductal glycoprotein 1, 120kDa

Search name

Human OVGP ELISA KIT ,Human OVGP1 ELISA KIT ,Human CHIT5 ELISA KIT ,Human EGP ELISA KIT ,Human MUC9 ELISA KIT ,Human OGP ELISA KIT ,Human estrogen-dependent oviduct protein ELISA KIT ,Human mucin 9 ELISA KIT ,Human mucin-9 ELISA KIT ,Human oviduct glycoprotein ELISA KIT ,Human oviduct-specific glycoprotein ELISA KIT ,Human oviductin ELISA KIT ,Human oviductal glycoprotein 1 120kDa ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Oviduct- specific glycoprotein, OVGP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Oviduct- specific glycoprotein, OVGP1, During the reaction, Human Oviduct- specific glycoprotein, OVGP1 in the sample or standard competes with a fixed amount of biotin-labeled Human Oviduct- specific glycoprotein, OVGP1 for sites on a pre-coated Monoclonal antibody specific to Human Oviduct- specific glycoprotein, OVGP1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Oviduct- specific glycoprotein, OVGP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.