Human PADI3 (Protein- arginine deiminase type- 3) ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Human Padi3 ELISA KIT ,Human PAD3 ELISA KIT ,Human PDI3 ELISA KIT ,Human peptidyl arginine deiminase ELISA KIT ,Human  type III ELISA KIT ,Human peptidylarginine deiminase III ELISA KIT ,Human protein-arginine deiminase type III ELISA KIT ,Human peptidyl arginine deiminase 3 ELISA KIT

Intended use

Human PADI3 ELISA kit allows for the in vitro quantitative determination of Human Protein- arginine deiminase type- 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Human PADI3 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PADI3, During the reaction, PADI3 in the sample or standard competes with a fixed amount of biotin-labeled PADI3 for sites on a pre-coated Monoclonal antibody specific to PADI3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PADI3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product    Min.Order 5kits

Delivery time 4-6month

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