Human PDLIM1 ELISA Kit
Product Name:Human PDLIM1 ELISA Kit
Packing:96T
Catalog No.:ELA-E3134h
Gene Name:PDLIM1
Detect Range:0.156-10 ng/mL
Sensitivity:0.03ng/mL
Target Protein Name:Human PDZ and LIM domain protein 1
Alternative Name:PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36, PDLIM1, CLIM1, CLP36
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human PDLIM1 ELISA Kit allows for the in vitro quantitative determination of Human PDZ and LIM domain protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human PDLIM1 ELISA Kit has been pre-coated with an PDLIM1 antibody specific to Human PDZ and LIM domain protein 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human PDZ and LIM domain protein 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PDZ and LIM domain protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human PDZ and LIM domain protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!