Human Phospho ser15 P53 ELISA KIT

$566.00

  • Model: EF007389
  • 100 Units in Stock
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Phospho ser15 P53 ELISA KIT|Human

Catalog  EF007389

Packing 48T/96T

Reactivity Human

Detect Range 62.5-4000pg/ml

Phospho ser15 P53 ELISA KIT Sensitivity< 37.5pg/ml

Application Phospho ser15 P53 ELISA KIT|Human  is for detecting the concentration of Human Phospho ser15 P53 in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

 

Principle of Phospho ser15 P53 ELISA KIT|Human

The microtiter plate provided in Phospho ser15 P53 ELISA KIT|Human has been pre-coated with an Human Phospho ser15 P53 antibody specific to Human Phospho ser15 P53. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Phospho ser15 P53antibody preparation specific for Human Phospho ser15 P53 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Phospho ser15 P53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Phospho ser15 P53in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

*Please noted that Phospho ser15 P53 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!

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