Human Pigment epithelium- derived factor precursor ELISA Kit

Product Name:Human Pigment epithelium- derived factor precursor ELISA Kit
Packing:96T

Catalog No.:ELA-E1972h

Gene Name:Pre-PEDF

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Pigment epithelium-derived factor precursor

Alternative Name:Pre-PEDF,Pigment epithelium-derived factor precursor
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Pigment epithelium- derived factor precursor ELISA Kit allows for the in vitro quantitative determination of Pigment epithelium-derived factor precursor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Pigment epithelium- derived factor precursor ELISA Kit has been pre-coated with an Pigment epithelium-derived factor precursor antibody specific to Pigment epithelium-derived factor precursor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Pigment epithelium-derived factor precursor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pigment epithelium-derived factor precursor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Pigment epithelium-derived factor precursor in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!