Human PKLR ELISA Kit

Product Name:Human PKLR ELISA Kit
Packing:96T

Catalog No.:ELA-E0208h

Gene Name:PKLR

Detect Range:3.12-200 U/L

Sensitivity:1.5U/L

Target Protein Name:Human Pyruvate kinase isozymes R/L

Alternative Name:Pyruvate kinase isozymes R/L, Pyruvate kinase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, PKLR, PK1, PKL, Red cell/liver pyruvate kinase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PKLR ELISA Kit allows for the in vitro quantitative determination of Human Pyruvate kinase isozymes R/L concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PKLR ELISA Kit has been pre-coated with an PKLR antibody specific to Human Pyruvate kinase isozymes R/L .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Pyruvate kinase isozymes R/L and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Pyruvate kinase isozymes R/L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm