Human PON1 ELISA Kit

Product Name:Human PON1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0243h

Gene Name:PON1

Detect Range:1.56-100 ng/mL

Sensitivity:0.78 ng/mL

Target Protein Name:Human Serum paraoxonase/arylesterase 1

Alternative Name:Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, A-esterase 1, K-45, Serum aryldialkylphosphatase 1, PON1, PON
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PON1 ELISA Kit allows for the in vitro quantitative determination of Human Serum paraoxonase/arylesterase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PON1 ELISA Kit has been pre-coated with an PON1 antibody specific to Human Serum paraoxonase/arylesterase 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Serum paraoxonase/arylesterase 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Serum paraoxonase/arylesterase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm