Human preptin ELISA Kit

Starting at: $696.00

  • Model: ELA-E0707h
  • 20 Units in Stock
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Human Preptin ELISA Kit

96 Tests

Operating instructions

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Preptin

 

Search name

Human Preptin ELISA Kit, Preptin ELISA Kit

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human preptin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to preptin, During the reaction, preptin in the sample or standard competes with a fixed amount of biotin-labeled preptin for sites on a pre-coated Monoclonal antibody specific to preptin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of preptin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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