Human PRKACA ELISA Kit

Product Name:Human PRKACA ELISA Kit
Packing:96T

Catalog No.:ELA-E1918h

Gene Name:PRKACA

Detect Range:0.78-50 ng/mL

Sensitivity:0.34ng/mL

Target Protein Name:Human cAMP-dependent protein kinase catalytic subunit alpha

Alternative Name:cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha, PRKACA, PKACA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PRKACA ELISA Kit allows for the in vitro quantitative determination of Human cAMP-dependent protein kinase catalytic subunit alpha concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PRKACA ELISA Kit has been pre-coated with an PRKACA antibody specific to Human cAMP-dependent protein kinase catalytic subunit alpha .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human cAMP-dependent protein kinase catalytic subunit alpha and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human cAMP-dependent protein kinase catalytic subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human cAMP-dependent protein kinase catalytic subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!