Human Probable fructose- 2,6- bisphosphatase TIGAR, TIGAR ELISA

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  • Model: ELI-22883h
  • 20 Units in Stock
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Human Probable fructose-2,6-bisphosphatase TIGAR, TIGAR ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

TIGAR,fructose-2,6-bisphosphatase TIGAR; C12orf5; FR2BP; Probable fructose-2,6-bisphosphatase TIGAR,TP53-induced glycolysis and apoptosis regulator; fructose-2,6-bisphosphate 2-phosphatase; transactivated by NS3TP2 protein; TP53 induced glycolysis regulatory phosphatase

 

Search name

Human TIGAR ELISA KIT ,Human fructose-2 ELISA KIT ,Human 6-bisphosphatase TIGAR ELISA KIT ,Human C12orf5 ELISA KIT ,Human FR2BP ELISA KIT ,Human Probable fructose-2 ELISA KIT ,Human 6-bisphosphatase TIGAR ELISA KIT ,Human TP53-induced glycolysis and apoptosis regulator ELISA KIT ,Human fructose-2 ELISA KIT ,Human 6-bisphosphate 2-phosphatase ELISA KIT ,Human transactivated by NS3TP2 protein ELISA KIT ,Human TP53 induced glycolysis regulatory phosphatase ELISA KIT

 

Intended use

Human TIGAR ELISA KIT allows for the in vitro quantitative determination of Human Probable fructose-2,6-bisphosphatase TIGAR concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Probable fructose-2,6-bisphosphatase TIGAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forProbable fructose-2,6-bisphosphatase TIGAR and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Probable fructose-2,6-bisphosphatase TIGAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Probable fructose-2,6-bisphosphatase TIGAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10.0ng/ml

PDF Manual download

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