Human Programmed cell death 1 ligand 1,CD274 ELISA Kit

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  • Model: ELA-E0513h
  • 20 Units in Stock
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Human Programmed cell death 1 ligand 1, CD274 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

CD274,CD274 molecule; CD274 antigen; B7-H; B7H1; B7-H1,PD-L1; PDCD1L1; PDCD1LG1; PDL1; B7 homolog 1; PDCD1 ligand 1; programmed cell death 1 ligand 1; programmed death ligand 1

 

Search name

Human CD274 ELISA KIT ,Human CD274 molecule ELISA KIT ,Human CD274 antigen ELISA KIT ,Human B7-H ELISA KIT ,Human B7H1 ELISA KIT ,Human B7-H1 ELISA KIT ,Human PD-L1 ELISA KIT ,Human PDCD1L1 ELISA KIT ,Human PDCD1LG1 ELISA KIT ,Human PDL1 ELISA KIT ,Human B7 homolog 1 ELISA KIT ,Human PDCD1 ligand 1 ELISA KIT ,Human programmed cell death 1 ligand 1 ELISA KIT ,Human programmed death ligand 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human programmed cell death 1 ligand 1,PDCD1 ligand 1 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDCD1 ligand 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDCD1 ligand 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDCD1 ligand 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDCD1 ligand 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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