Human Protein arginine N- methyltransferase 1, Prmt1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Search name
Human Prmt1 ELISA KIT ,Human HMT2 ELISA KIT, Human IR1B ELISA KIT ,Human Mrmt1 ELISA KIT ,Human ANM1 ELISA KIT ,Human HCP1 ELISA KIT ,Human HRMT1L2 ELISA KIT ,Human IR1B4 ELISA KIT ,Human HMT1 (hnRNP methyltransferase ELISA KIT ,Human S. cerevisiae)-like 2 ELISA KIT ,Human heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2 ELISA KIT ,Human interferon receptor 1-bound protein 4 ELISA KIT ,Human protein arginine methyltransferase 1 ELISA KIT ,Human histone-arginine N-methyltransferase PRMT1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Protein arginine N-methyltransferase 1, Prmt1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Protein arginine N-methyltransferase 1,Prmt1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Protein arginine N-methyltransferase 1,Prmt1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein arginine N-methyltransferase 1,Prmt1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Protein arginine N-methyltransferase 1,Prmt1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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