Human Protocadherin alpha- 8, PCDHA8 ELISA KIT
Product Name:Human Protocadherin alpha- 8, PCDHA8 ELISA KIT
Packing:96T
Catalog No.:ELI-22836h
Gene Name:PCDHA8
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Protocadherin alpha-8(PCDHA8
Alternative Name:PCDHA8,Protocadherin alpha-8(PCDHA8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protocadherin alpha- 8, PCDHA8 ELISA KIT allows for the in vitro quantitative determination of Protocadherin alpha-8(PCDHA8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protocadherin alpha- 8, PCDHA8 ELISA KIT has been pre-coated with an Protocadherin alpha-8(PCDHA8 antibody specific to Protocadherin alpha-8(PCDHA8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protocadherin alpha-8(PCDHA8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protocadherin alpha-8(PCDHA8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protocadherin alpha-8(PCDHA8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!