Human Psychosine receptor, GPR65 ELISA KIT
Product Name:Human Psychosine receptor, GPR65 ELISA KIT
Packing:96T
Catalog No.:ELI-16793h
Gene Name:GPR65
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Psychosine receptor(GPR65
Alternative Name:GPR65,Psychosine receptor(GPR65
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Psychosine receptor, GPR65 ELISA KIT allows for the in vitro quantitative determination of Psychosine receptor(GPR65 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Psychosine receptor, GPR65 ELISA KIT has been pre-coated with an Psychosine receptor(GPR65 antibody specific to Psychosine receptor(GPR65 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Psychosine receptor(GPR65 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Psychosine receptor(GPR65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Psychosine receptor(GPR65 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!