Human Putative microRNA 17 host gene protein, MIR17HG ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MIR17HG,C13orf25; FGLDS2; LINC00048; MIHG1; MIRH1; MIRHG1; NCRNA00048; miR-17-92; miR-17-92 cluster host gene (non-protein coding)

Search name

Human MIR17HG ELISA KIT ,Human C13orf25 ELISA KIT ,Human FGLDS2 ELISA KIT ,Human LINC00048 ELISA KIT ,Human MIHG1 ELISA KIT ,Human MIRH1 ELISA KIT ,Human MIRHG1 ELISA KIT ,Human NCRNA00048 ELISA KIT ,Human miR-17-92 ELISA KIT ,Human miR-17-92 cluster host gene ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Putative microRNA 17 host gene protein, MIR17HG beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Putative microRNA 17 host gene protein, MIR17HG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Putative microRNA 17 host gene protein, MIR17HG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Putative microRNA 17 host gene protein, MIR17HG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Putative microRNA 17 host gene protein, MIR17HG in the samples is then determined by comparing the O.D. of the samples to the standard curve.