Human Pyruvate Kinase, M2-PK ELISA Kit


  • Model: ELI-02114h
  • 50 Units in Stock
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Human Pyruvate Kinase, M2-PK ELISA Kit

ELI-02114h    96tests

Intended use
Human Pyruvate Kinase, M2-PK ELISA Kit allows for the in vitro quantitative determination of human Pyruvate Kinase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in Human Pyruvate Kinase, M2-PK ELISA Kit has been pre-coated with an antibody specific to Pyruvate Kinase2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pyruvate Kinase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyruvate Kinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pyruvate Kinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human Pyruvate Kinase ELISA KIT Range 0.78-50 U/mL

1.  This product is FOR RESEARCH USE ONLY!

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