Human RC3H1 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name RING finger and C3H zinc finger protein 1
Protein Name leukotriene A4 hydrolase
Alternative Name
Human RC3H1 ELISA KIT,Human RNF198 ELISA KIT ,Human ROQUIN ELISA KIT ,Human roquin-1 ELISA KIT ,Human RING finger and C3H zinc finger protein 1 ELISA KIT ,Human RING finger and CCCH-type zinc finger domain-containing protein 1 ELISA KIT ,Human RING finger protein 198 ELISA KIT ,Human ring finger and CCCH-type domains 1 ELISA KIT
Intended use
Human RC3H1 ELISA KIT allows for the in vitro quantitative determination of RC3H1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Human RC3H1 ELISA KIT has been pre-coated with an RC3H1 antibody specific to RC3H1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for RC3H1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RC3H1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RC3H1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
