Human Roundabout homolog 1,ROBO1 ELISA KIT

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  • Model: ELI-53163h
  • 20 Units in Stock
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Human Roundabout homolog 1, ROBO1 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Roundabout homolog 1,Deleted in U twenty twenty,H-Robo-1,ROBO1,DUTT1, ROBO1,DUTT1; SAX3; deleted in U twenty twenty; roundabout homolog 1; roundabout axon guidance receptor homolog 1

 

Search name

Human Roundabout homolog 1 ELISA KIT ,Human Deleted in U twenty twenty ELISA KIT ,Human H-Robo-1 ELISA KIT ,Human ROBO1 ELISA KIT ,Human DUTT1 ELISA KIT ,Human ROBO1 ELISA KIT ,Human DUTT1 ELISA KIT ,Human SAX3 ELISA KIT ,Human deleted in U twenty twenty ELISA KIT ,Human roundabout homolog 1 ELISA KIT ,Human roundabout axon guidance receptor homolog 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Roundabout homolog 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Roundabout homolog 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Roundabout homolog 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Roundabout homolog 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Roundabout homolog 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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