Human S100P ELISA Kit
Product Name:Human S100P ELISA Kit
Packing:96T
Catalog No.:ELA-E2054h
Gene Name:S100P
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Human Protein S100-P
Alternative Name:Protein S100-P, Protein S100-E, S100P, S100E, MIG9, S100 calcium-binding protein P
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human S100P ELISA Kit allows for the in vitro quantitative determination of Human Protein S100-P concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human S100P ELISA Kit has been pre-coated with an S100P antibody specific to Human Protein S100-P .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein S100-P and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein S100-P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Protein S100-P in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!