Human Semaphorin- 3C ELISA Kit

Product Name:Human Semaphorin- 3C ELISA Kit
Packing:96T

Catalog No.:ELA-E2271h

Gene Name:SM(SEMA-3C

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Semaphorin-3C

Alternative Name:SM(SEMA-3C,Semaphorin-3C
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Semaphorin- 3C ELISA Kit allows for the in vitro quantitative determination of Semaphorin-3C concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Semaphorin- 3C ELISA Kit has been pre-coated with an Semaphorin-3C antibody specific to Semaphorin-3C .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Semaphorin-3C and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Semaphorin-3C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Semaphorin-3C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!