Human Serine/ threonine- protein kinase SIK2, SIK2 ELISA KIT

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  • Model: ELI-39362h
  • 20 Units in Stock
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Human Serine/ threonine-protein kinase SIK2, SIK2 ELISA KIT

Alternative name

Human SIK2 ELISA KIT ,Human Class 2.7.11.1 ELISA KIT ,Human Serine/threonine-protein kinase SIK2 ELISA KIT ,Human Serine/threonine-protein kinase SNF1-like kinase 2 ELISA KIT ,Human Qin-induced kinase ELISA KIT ,Human Salt-inducible protein kinase 2 ELISA KIT ,Human SIK-2 ELISA KIT ,Human serine/threonine-protein kinase SIK2 ELISA KIT ,Human LOH11CR1I ELISA KIT ,Human QIK ELISA KIT ,Human SNF1LK2 ELISA KIT ,Human SIK-2 ELISA KIT ,Human SNF1-like kinase 2 ELISA KIT ,Human qin-induced kinase ELISA KIT ,Human salt-inducible protein kinase 2 ELISA KIT ,Human salt-inducible serine/threonine kinase 2 ELISA KIT ,Human serine/threonine-protein kinase SNF1-like kinase 2 ELISA KIT ,Human salt-inducible kinase 2 ELISA KIT

 

Intended use

Human SIK2 ELISA KIT allows for the in vitro quantitative determination of human Serine/threonine-protein kinase SIK2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/threonine-protein kinase SIK2, During the reaction, Serine/threonine-protein kinase SIK2 in the sample or standard competes with a fixed amount of biotin-labeled Serine/threonine-protein kinase SIK2 for sites on a pre-coated Monoclonal antibody specific to Serine/threonine-protein kinase SIK2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serine/threonine-protein kinase SIK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.31-20.0ng/ml

PDF Manual download

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