Human serum amyloid A3, SAA3 ELISA Kit

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  • Model: ELA-E1795h
  • 20 Units in Stock
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Human serum amyloid A3, SAA3 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Putative serum amyloid A-3 protein,Serum amyloid A-3 protein,SAA3,SAA3P

 

Search name

Human Putative serum amyloid A-3 protein ELISA KIT ,Human Serum amyloid A-3 protein ELISA KIT ,Human SAA3 ELISA KIT ,Human SAA3P ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human putative serum amyloid a-3 protein,Putative serum amyloid A-3 protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Putative serum amyloid A-3 protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Putative serum amyloid A-3 protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Putative serum amyloid A-3 protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Putative serum amyloid A-3 protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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