Human Sestrin 3, SESN3 ELISA kit

Starting at: $696.00

  • Model: ELA-E12181h
  • 20 Units in Stock
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Human Sestrin-3, SESN3 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Sestrin-3,SESN3,SEST3; sestrin-3; sestrin 3

 

Search name

Human Sestrin-3 ELISA KIT ,Human SESN3 ELISA KIT ,Human SEST3 ELISA KIT ,Human sestrin-3 ELISA KIT ,Human sestrin 3 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human sestrin-3,Sestrin-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sestrin-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Sestrin-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sestrin-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Sestrin-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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