Human Soluble protein- 100 ELISA Kit

Starting at: $696.00

  • Model: ELA-E0568h
  • 20 Units in Stock
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Human Soluble protein-100 ELISA Kit


96 Tests


Alternative name

Human Soluble protein-100 ELISA Kit,Human S100 ELISA KIT ,Human S-100 ELISA KIT ,Human soluble S100 calcium binding protein A1 ELISA KIT ,Human soluble S100 protein ELISA KIT ,Human soluble alpha polypeptide ELISA KIT ,Human soluble S100 calcium-binding protein A1 ELISA KIT ,Human solubleS100 alpha ELISA KIT ,Human soluble S100-alpha ELISA KIT ,Human soluble S100A ELISA KIT ,Human solubleS-100 protein alpha chain ELISA KIT ,Human solubleS-100 protein subunit alpha ELISA KIT ,


Intended use

Human Soluble protein-100 ELISA Kit allows for the in vitro quantitative determination of Human S-100 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.


Test principle

The microtiter plate provided in Human Soluble protein-100 ELISA Kit has been pre-coated with an antibody specific to S-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain S-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 78-5000pg/ml

Human Soluble protein-100 ELISA Kit Manual download

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