Human soluble Urokinase- type Plasminogen Activator Receptor ELI

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  • Model: ELA-E14888h
  • 20 Units in Stock
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Human Soluble Urokinase plasminogen activator surface receptor,SuPAR ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY!

 

Synonyms

sPLAUR,sMO,sCD87,soluble Urokinase plasminogen activator surface receptor,sU-PAR,suPAR, soluble Monocyte activation antigen Mo3,sURKR; soluble u-plasminogen activator receptor form 2; soluble urokinase plasmin

 

Search name

Human sPLAUR ELISA KIT ,Human sMO ELISA KIT ,Human sCD87 ELISA KIT ,Human soluble Urokinase plasminogen activator surface receptor ELISA KIT ,Human sU-PAR ELISA KIT ,Human suPAR ELISA KIT ,Human  soluble Monocyte activation antigen Mo3 ELISA KIT ,Human sURKR ELISA KIT ,Human soluble u-plasminogen activator receptor form 2 ELISA KIT ,Human soluble urokinase plasmin ELISA KIT

 

Intended use

Human sPLAUR ELISA KIT allows for the in vitro quantitative determination of human soluble urokinase plasminogen activator surface receptor,suPAR concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Human sPLAUR ELISA KIT has been pre-coated with an antibody specific to uPAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for uPAR and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain uPAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of uPAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10ng/ml

PDF Manual download

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