Human Sphingosine 1 Phosphate Receptor 1 ELISA Kit

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  • Model: ELA-E9909h
  • 20 Units in Stock
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Human Sphingosine 1- phosphate receptor 1, S1PR1 ELISA KIT

96 Tests

Operating instructions

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Sphingosine 1-phosphate receptor 1(S1P receptor 1,S1P1),;

Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1(S1P receptor Edg-1),;

 

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of human sphingosine 1-phosphate receptor 1,S1P receptor 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P receptor 1, During the reaction, S1P receptor 1 in the sample or standard competes with a fixed amount of biotin-labeled S1P receptor 1 for sites on a pre-coated Monoclonal antibody specific to S1P receptor 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S1P receptor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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