Human Sphingosine 1 Phosphate Receptor 5, S1PR5 ELISA kit

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  • Model: ELA-E11210h
  • 20 Units in Stock
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Human Sphingosine 1 Phosphate Receptor 5, S1PR5 ELISA kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Sphingosine 1-phosphate receptor 5(S1P receptor 5,S1P5),;

Endothelial differentiation G-protein-coupled receptor 8,Sphingosine 1-phosphate receptor Edg-8(S1P receptor Edg-8),;

 

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of human sphingosine 1-phosphate receptor 5,S1P receptor 5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P receptor 5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S1P receptor 5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain S1P receptor 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S1P receptor 5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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