Human Sphingosine, SPH ELISA Kit

96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Sphingosine,; 2-amino-4-octadecene-1,3-diol

Search name
Human Sphingosine ELISA Kit, Human 2-amino-4-octadecene-1,3-diol ELISA Kit, Human Sph ELISA Kit, Sphingosine ELISA Kit,
2-amino-4-octadecene-1,3-diol ELISA Kit, Sph ELISA Kit

Intended use
Human Sphingosine ELISA KIT allows for the in vitro quantitative determination of Sphingosine concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
Human Sphingosine ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Sphingosine, During the reaction, Sphingosine in the sample or standard competes with a fixed
amount of biotin-labeled Sphingosine for sites on a pre-coated Monoclonal antibody specific to Sphingosine. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Sphingosine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human Sphingosine ELISA KIT Range 0.15-10ng/mL

Human Sphingosine ELISA KIT PDF manual download