Human soluble receptor activator of nuclear factor- kB ligand, sRANKL ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Tumor necrosis factor ligand superfamily member 11,TNF-related activation-induced cytokine(TRANCE),Receptor activator of nuclear
factor kappa-B ligand(RANKL),Osteoclast differentiation factor(ODF),Osteoprotegerin ligand(OPGL),
CD254,ODF,OPGL,OPTB2,RANKL,TRANCE,hRANKL2,sOdf, TNF-related activation-induced cytokine, osteoclast differentiation factor,
osteoprotegerin ligand, receptor activator of nuclear factor kappa B ligand, receptor activator of nuclear factor kappa-B ligand, tumor
necrosis factor ligand superfamily member 11,Opgl,Rankl,Trance

Search name
Human Tnfsf11 Elisa kit, Human Rankl ELISA Kit,Human opgl ELISA Kit,Human Trance ELISA Kit, Tnfsf11 Elisa kit, Rankl ELISA
Kit,opgl ELISA Kit,Trance ELISA Kit, Human Tumor necrosis factor ligand superfamily member 11 ELISA Kit, Tumor necrosis
factor ligand superfamily member 11 ELISA Kit,Human ODF Elisa kit, receptor activator of nuclear factor kappa B ligand ELISA
KIT,Human receptor activator of nuclear factor kappa B ligand ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human tumor necrosis factor ligand superfamily member
11,Tumor necrosis factor ligand superfamily member 11 concentrations in serum, Plasma, tissue homogenates and Cell culture
supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor necrosis factor ligand superfamily member
11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation
specific for Tumor necrosis factor ligand superfamily member 11 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to
each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tumor necrosis
factor ligand superfamily member 11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tumor necrosis factor ligand superfamily member 11 in
the samples is then determined by comparing the O.D. of the samples to the standard curve.

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