Human SRD5A2 ELISA KIT

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  • Model: ELI-53179h
  • 20 Units in Stock
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Human 3- oxo- 5- alpha- steroid 4- dehydrogenase 2, Srd5a2 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Srd5a2,5 alpha-SR2; S5AR 2; SR type 2; steroid 5-alpha-reductase 2; type II 5-alpha reductase; steroid-5-alpha-reductase alpha polypeptide 2 ,3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2,Steroid 5-alpha-reductase 2,S5AR 2,5 alpha-SR2,SR type 2,

 

Search name

Human Srd5a2 ELISA KIT ,Human 5 alpha-SR2 ELISA KIT ,Human S5AR 2 ELISA KIT ,Human SR type 2 ELISA KIT ,Human steroid 5-alpha-reductase 2 ELISA KIT ,Human type II 5-alpha reductase ELISA KIT ,Human steroid-5-alpha-reductase alpha polypeptide 2  ELISA KIT ,Human 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2 ELISA KIT ,Human  3-oxo-5-alpha-steroid 4-dehydrogenase 2 ELISA KIT ,Human Steroid 5-alpha-reductase 2 ELISA KIT ,Human S5AR 2 ELISA KIT ,Human 5 alpha-SR2 ELISA KIT ,Human SR type 2 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2 ,3-oxo-5-alpha-steroid 4-dehydrogenase 2concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-oxo-5-alpha-steroid 4-dehydrogenase 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for3-oxo-5-alpha-steroid 4-dehydrogenase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 3-oxo-5-alpha-steroid 4-dehydrogenase 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 3-oxo-5-alpha-steroid 4-dehydrogenase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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