Human SREBF2 ELISA Kit

Product Name:Human SREBF2 ELISA Kit
Packing:96T

Catalog No.:ELA-E12196h

Gene Name:SREBF2

Detect Range:0.312-20 ng/mL

Sensitivity:0.12ng/ml

Target Protein Name:Human Sterol regulatory element-binding protein 2

Alternative Name:Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, bHLHd2, Sterol regulatory element-binding transcription factor 2, SREBF2, BHLHD2, SREBP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human SREBF2 ELISA Kit allows for the in vitro quantitative determination of Human Sterol regulatory element-binding protein 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human SREBF2 ELISA Kit has been pre-coated with an SREBF2 antibody specific to Human Sterol regulatory element-binding protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Sterol regulatory element-binding protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Sterol regulatory element-binding protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Sterol regulatory element-binding protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!