Human SRF ELISA Kit

Product Name:Human SRF ELISA Kit
Packing:96T

Catalog No.:ELA-E6270h

Gene Name:SRF

Detect Range:31.2-2000 pg/mL

Sensitivity:13pg/mL

Target Protein Name:Human Serum response factor

Alternative Name:Serum response factor, SRF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human SRF ELISA Kit allows for the in vitro quantitative determination of Human Serum response factor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human SRF ELISA Kit has been pre-coated with an SRF antibody specific to Human Serum response factor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Serum response factor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Serum response factor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Serum response factor in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!