Human Steroid 5 Alpha Reductase 1, SRD5A1 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human SRD5A1 ELISA KIT ,Human S5AR 1 ELISA KIT ,Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA KIT ,Human SR type 1 ELISA KIT ,Human steroid 5-alpha-reductase type I ELISA KIT ,Human steroid-5-alpha-reductase ELISA KIT ,Human alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) ELISA KIT ,Human steroid 5 alpha-reductase 1 ELISA KIT

Intended use

Human SRD5A1 ELISA kit allows for the in vitro quantitative determination of Human SRD5A1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Human SRD5A1 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SRD5A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SRD5A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SRD5A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human SRD5A1 ELISA kit  Range 0.15-10ng/ml

Human SRD5A1 ELISA kit  Manual

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