Human SULT1A2 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name SULT1A2
Protein Name Sulfotransferase 1A2
Range 0.156-10ng/ml
Alternative Name
Human SULT1A2 ELISA KIT ,Human HAST4 ELISA KIT ,Human P-PST ELISA KIT ,Human ST1A2 ELISA KIT ,Human STP2 ELISA KIT ,Human TSPST2 ELISA KIT ,Human sulfotransferase 1A2 ELISA KIT ,Human P-PST 2 ELISA KIT ,Human aryl sulfotransferase 2 ELISA KIT ,Human arylamine sulfotransferase ELISA KIT ,Human phenol sulfotransferase 2 ELISA KIT ,Human phenol-preferring phenol sulfotransferase2 ELISA KIT ,Human phenol-sulfating phenol sulfotransferase 2 ELISA KIT ,Human phenolic-metabolizing(P) form of PST ELISA KIT ,Human sulfotransferase family ELISA KIT ,Human cytosolic ELISA KIT ,Human 1A ELISA KIT ,Human phenol-preferring ELISA KIT ,Human member 2 ELISA KIT ,Human thermostable phenol sulfotransferase ELISA KIT ,Human sulfotransferase family 1A member 2 ELISA KIT
Intended use
Human SULT1A2 ELISA KIT allows for the in vitro quantitative determination of SULT1A2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Human SULT1A2 ELISA KIT has been pre-coated with an SULT1A2 antibody specific to SULT1A2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SULT1A2 and then avidin conjugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SULT1A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SULT1A2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum -Use a serum separator tube(SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20