Human T- cell acute lymphoblastic leukemia antigen ELISA KIT
Product Name:Human T- cell acute lymphoblastic leukemia antigen ELISA KIT
Packing:96T
Catalog No.:ELA-E0751h
Gene Name:TALLA-1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:T-cell acute lymphoblastic leukemia antigen
Alternative Name:TALLA-1,T-cell acute lymphoblastic leukemia antigen
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human T- cell acute lymphoblastic leukemia antigen ELISA KIT allows for the in vitro quantitative determination of T-cell acute lymphoblastic leukemia antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human T- cell acute lymphoblastic leukemia antigen ELISA KIT has been pre-coated with an T-cell acute lymphoblastic leukemia antigen antibody specific to T-cell acute lymphoblastic leukemia antigen .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for T-cell acute lymphoblastic leukemia antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain T-cell acute lymphoblastic leukemia antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of T-cell acute lymphoblastic leukemia antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!