Human Tacrolimus FK506 ELISA Kit

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  • Model: ELA-E1207h
  • 20 Units in Stock
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Human Tacrolimus FK506 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Tacrolimus,fujimycin, FK-506, fujimycin, Prograf, Advagraf, Protopic

Search name
Human Tacrolimus ELISA Kit, Human fujimycin ELISA Kit, Human FK-506 ELISA Kit, Human Prograf ELISA Kit, Human Advagraf ELISA Kit, Human Protopic ELISA Kit

Intended use
Human Tacrolimus FK506 ELISA Kit allows for the in vitro quantitative determination of Human tacrolimus,Tacrolimus concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in Human Tacrolimus FK506 ELISA Kit has been pre-coated with an antibody specific to Tacrolimus. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tacrolimus and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Tacrolimus, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tacrolimus in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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