Human Thymosin beta- 4, TMSB4X ELISA KIT
Product Name:Human Thymosin beta- 4, TMSB4X ELISA KIT
Packing:96T
Catalog No.:ELI-28466h
Gene Name:TMSB4X
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Thymosin beta-4(TMSB4X
Alternative Name:TMSB4X,Thymosin beta-4(TMSB4X
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Thymosin beta- 4, TMSB4X ELISA KIT allows for the in vitro quantitative determination of Thymosin beta-4(TMSB4X concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Thymosin beta- 4, TMSB4X ELISA KIT has been pre-coated with an Thymosin beta-4(TMSB4X antibody specific to Thymosin beta-4(TMSB4X .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Thymosin beta-4(TMSB4X and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Thymosin beta-4(TMSB4X, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Thymosin beta-4(TMSB4X in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!