Human tissue polypeptide specific antigen ELISA Kit
Product Name:Human tissue polypeptide specific antigen ELISA Kit
Packing:96T
Catalog No.:ELA-E1281h
Gene Name:TPS
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:tissue polypeptide specific antigen
Alternative Name:TPS,tissue polypeptide specific antigen
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human tissue polypeptide specific antigen ELISA Kit allows for the in vitro quantitative determination of tissue polypeptide specific antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human tissue polypeptide specific antigen ELISA Kit has been pre-coated with an tissue polypeptide specific antigen antibody specific to tissue polypeptide specific antigen .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for tissue polypeptide specific antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain tissue polypeptide specific antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of tissue polypeptide specific antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!