Human TNFSF14 ELISA Kit

Product Name:Human TNFSF14 ELISA Kit
Packing:96T

Catalog No.:ELA-E2060h

Gene Name:TNFSF14

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Human Tumor necrosis factor ligand superfamily member 14

Alternative Name:Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L, Herpesvirus entry mediator ligand, TNFSF14, HVEML, LIGHT, CD258
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human TNFSF14 ELISA Kit allows for the in vitro quantitative determination of Human Tumor necrosis factor ligand superfamily member 14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human TNFSF14 ELISA Kit has been pre-coated with an TNFSF14 antibody specific to Human Tumor necrosis factor ligand superfamily member 14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Tumor necrosis factor ligand superfamily member 14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Tumor necrosis factor ligand superfamily member 14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Tumor necrosis factor ligand superfamily member 14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!