Human Transcription factor 7, TCF7 ELISA KIT
Product Name:Human Transcription factor 7, TCF7 ELISA KIT
Packing:96T
Catalog No.:ELI-52101h
Gene Name:TCF7
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Transcription factor 7(TCF7
Alternative Name:TCF7,Transcription factor 7(TCF7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Transcription factor 7, TCF7 ELISA KIT allows for the in vitro quantitative determination of Transcription factor 7(TCF7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Transcription factor 7, TCF7 ELISA KIT has been pre-coated with an Transcription factor 7(TCF7 antibody specific to Transcription factor 7(TCF7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Transcription factor 7(TCF7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transcription factor 7(TCF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Transcription factor 7(TCF7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!