Human Transcription factor Sp1,SP1 ELISA KIT

Starting at: $696.00

  • Model: ELI-30143h
  • 20 Units in Stock
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Human Transcription factor Sp1, SP1 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Sp1,Sp1 transcription factor; transcription factor Sp1; specificity protein 1

 

Search name

Human Sp1 ELISA KIT ,Human Sp1 transcription factor ELISA KIT ,Human transcription factor Sp1 ELISA KIT ,Human specificity protein 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Transcription factor Sp1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription factor Sp1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Transcription factor Sp1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transcription factor Sp1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Transcription factor Sp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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