Human Trefoil factor 1, TFF1 ELISA KIT

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  • Model: ELI-03393h
  • 20 Units in Stock
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Human Trefoil factor 1, TFF1 ELISA KIT

96 Tests

Operating instructions

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Trefoil factor 1,Protein pS2,Polypeptide P1.A,hP1.A,Breast cancer estrogen-inducible protein,PNR-2,TFF1,BCEI; D21S21; HP1.A; HPS2; pNR-2; pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; gastrointestinal trefoil protein pS2; polypeptide P1.A; protein pS2; trefoil factor 1

 

Search name

Human Trefoil factor 1 ELISA KIT ,Human Protein pS2 ELISA KIT ,Human Polypeptide P1.A ELISA KIT ,Human hP1.A ELISA KIT ,Human Breast cancer estrogen-inducible protein ELISA KIT ,Human PNR-2 ELISA KIT ,Human TFF1 ELISA KIT ,Human BCEI ELISA KIT ,Human D21S21 ELISA KIT ,Human HP1.A ELISA KIT ,Human HPS2 ELISA KIT ,Human pNR-2 ELISA KIT ,Human pS2 ELISA KIT ,Human breast cancer estrogen-inducible protein ELISA KIT ,Human breast cancer estrogen-inducible sequence ELISA KIT ,Human gastrointestinal trefoil protein pS2 ELISA KIT ,Human polypeptide P1.A ELISA KIT ,Human protein pS2 ELISA KIT ,Human trefoil factor 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Trefoil factor 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil factor 1, During the reaction, Trefoil factor 1 in the sample or standard competes with a fixed amount of biotin-labeled Trefoil factor 1 for sites on a pre-coated Monoclonal antibody specific to Trefoil factor 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Trefoil factor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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