Human Type II iodothyronine deiodinase, DIO2 ELISA KIT

Product Name:Human Type II iodothyronine deiodinase, DIO2 ELISA KIT
Packing:96T

Catalog No.:ELI-02065h

Gene Name:DIO2

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Type II iodothyronine deiodinase(DIO2

Alternative Name:DIO2,Type II iodothyronine deiodinase(DIO2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Type II iodothyronine deiodinase, DIO2 ELISA KIT allows for the in vitro quantitative determination of Type II iodothyronine deiodinase(DIO2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Type II iodothyronine deiodinase, DIO2 ELISA KIT has been pre-coated with an Type II iodothyronine deiodinase(DIO2 antibody specific to Type II iodothyronine deiodinase(DIO2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Type II iodothyronine deiodinase(DIO2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Type II iodothyronine deiodinase(DIO2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Type II iodothyronine deiodinase(DIO2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!