UBE2B ELISA KIT|Human
Catalog EF003993
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
UBE2B ELISA KIT Sensitivity< 0.188ng/ml
Application UBE2B ELISA KIT|Human is for detecting the concentration of Human UBE2B in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of UBE2B ELISA KIT|Human
The microtiter plate provided in UBE2B ELISA KIT|Human has been pre-coated with an Human UBE2B antibody specific to Human UBE2B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human UBE2Bantibody preparation specific for Human UBE2B then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human UBE2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human UBE2Bin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that UBE2B ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!