Human UBE2M(NEDD8-conjugating enzyme Ubc12)ELISA Kit

Starting at: $696.00

  • Model: ELI-17007h
  • 20 Units in Stock
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Human UBE2M (NEDD8- conjugating enzyme Ubc12) ELISA Kit (CUSTOM)

96 Tests

Operating instructions




Alternative name

Human UBE2M ELISA KIT ,Human UBC-RS2 ELISA KIT ,Human UBC12 ELISA KIT ,Human hUbc12 ELISA KIT ,Human NEDD8 carrier protein ELISA KIT ,Human NEDD8 protein ligase ELISA KIT ,Human ubiquitin carrier protein M ELISA KIT ,Human ubiquitin conjugating enzyme E2M ELISA KIT ,Human ubiquitin-conjugating enzyme E2M (UBC12 homolog ELISA KIT ,Human  yeast) ELISA KIT ,Human ubiquitin-protein ligase M ELISA KIT ,Human yeast UBC12 homolog ELISA KIT ,Human ubiquitin conjugating enzyme E2 M ELISA KIT


Intended use

Human UBE2M ELISA kit allows for the in vitro quantitative determination of Human NEDD8- conjugating enzyme Ubc12 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.


Test principle

Human UBE2M ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UBE2M, During the reaction, UBE2M in the sample or standard competes with a fixed amount of biotin-labeled UBE2M for sites on a pre-coated Monoclonal antibody specific to UBE2M. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UBE2M in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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