Human UBE4A(Ubiquitin conjugation factor E4 A)ELISA Kit

Starting at: $696.00

  • Model: ELI-51351h
  • 20 Units in Stock
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Human UBE4A (Ubiquitin conjugation factor E4 A) ELISA Kit (CUSTOM)

96 Tests

Operating instructions




Alternative name

Human UBE4A ELISA KIT ,Human UBOX2 ELISA KIT ,Human UFD2 ELISA KIT ,Human ubiquitination factor E4A (UFD2 homolog yeast) ELISA KIT ,Human ubiquitination factor E4 ELISA KIT


Intended use

Human UBE4A ELISA kit allows for the in vitro quantitative determination of Human Ubiquitin conjugation factor E4 A concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.


Test principle

Human UBE4A ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UBE4A, During the reaction, UBE4A in the sample or standard competes with a fixed amount of biotin-labeled UBE4A for sites on a pre-coated Monoclonal antibody specific to UBE4A. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UBE4A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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