Human Ubiquitin carboxyl- terminal hydrolase 47, USP47 ELISA KIT

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  • Model: ELI-17073h
  • 20 Units in Stock
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Human Ubiquitin carboxyl- terminal hydrolase 47, USP47 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

USP47,TRFP; Trf (TATA binding protein-related factor)-proximal homolog; deubiquitinating enzyme 47; ubiquitin carboxyl-terminal hydrolase 47; ubiquitin specific protease 47; ubiquitin thioesterase 47; ubiquitin thiolesterase 47; ubiquitin-specific-processing protease 47; ubiquitin specific peptidase 47

 

Search name

Human USP47 ELISA KIT ,Human TRFP ELISA KIT ,Human Trf (TATA binding protein-related factor)-proximal homolog ELISA KIT ,Human deubiquitinating enzyme 47 ELISA KIT ,Human ubiquitin carboxyl-terminal hydrolase 47 ELISA KIT ,Human ubiquitin specific protease 47 ELISA KIT ,Human ubiquitin thioesterase 47 ELISA KIT ,Human ubiquitin thiolesterase 47 ELISA KIT ,Human ubiquitin-specific-processing protease 47 ELISA KIT ,Human ubiquitin specific peptidase 47 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Ubiquitin carboxyl- terminal hydrolase 47, USP47 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin carboxyl- terminal hydrolase 47. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin carboxyl- terminal hydrolase 47 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl- terminal hydrolase 47, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ubiquitin carboxyl- terminal hydrolase 47 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

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Delivery time 4-6month

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