Human UCN3 ELISA Kit
Product Name:Human UCN3 ELISA Kit
Packing:96T
Catalog No.:ELA-E0072h
Gene Name:UCN3
Detect Range:0.156-10 ng/mL
Sensitivity:0.056ng/mL
Target Protein Name:Human Urocortin-3
Alternative Name:Urocortin-3, Stresscopin, Urocortin III, Ucn III, UCN3, SPC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human UCN3 ELISA Kit allows for the in vitro quantitative determination of Human Urocortin-3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human UCN3 ELISA Kit has been pre-coated with an UCN3 antibody specific to Human Urocortin-3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Urocortin-3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Urocortin-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm