Human UGP2 ELISA KIT

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  • Model: ELI-28569h
  • 20 Units in Stock
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Human UTP- - glucose- 1- phosphate uridylyltransferase, UGP2 ELISA Kit

96 Tests

Operating instructions

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

UGP2,UDPG; UDPGP; UDPGP2; UGP1; UGPP1; UGPP2; pHC379; UDP-glucose diphosphorylase; UDP-glucose pyrophosphorylase 1; UGPase 2; UTP--glucose-1-phosphate uridylyltransferase; UTP--glucose-1-phosphate uridylyltransferase 2; UTP-glucose-1-phosphate uridyltransferase; Uridyl diphosphate glucose pyrophosphorylase-1; uridyl diphosphate glucose pyrophosphorylase 2; UDP-glucose pyrophosphorylase 2

 

Search name

Human UGP2 ELIAS KIT,Human UDPG ELIAS KIT,Human UDPGP ELIAS KIT,Human UDPGP2 ELIAS KIT,Human UGP1 ELIAS KIT,Human UGPP1 ELIAS KIT,Human UGPP2 ELIAS KIT,Human pHC379 ELIAS KIT,Human UDP-glucose diphosphorylase ELIAS KIT,Human UDP-glucose pyrophosphorylase 1 ELIAS KIT,Human UGPase 2 ELIAS KIT,Human UTP--glucose-1-phosphate uridylyltransferase ELIAS KIT,Human UTP--glucose-1-phosphate uridylyltransferase 2 ELIAS KIT,Human UTP-glucose-1-phosphate uridyltransferase ELIAS KIT,Human Uridyl diphosphate glucose pyrophosphorylase-1 ELIAS KIT,Human uridyl diphosphate glucose pyrophosphorylase 2 ELIAS KIT,Human UDP-glucose pyrophosphorylase 2 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human UTP- - glucose- 1- phosphate uridylyltransferase, UGP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP-glucose pyrophosphorylase, During the reaction, UDP-glucose pyrophosphorylase in the sample or standard competes with a fixed amount of biotin-labeled UDP-glucose pyrophosphorylase for sites on a pre-coated Monoclonal antibody specific to UDP-glucose pyrophosphorylase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UDP-glucose pyrophosphorylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

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